Journal: PLoS ONE
Article Title: A Functional Genomics Approach to Establish the Complement of Carbohydrate Transporters in Streptococcus pneumoniae
doi: 10.1371/journal.pone.0033320
Figure Lengend Snippet: The relative metabolic activity of EI mutants of G54 (panel A) and DP1004 (panel B) was determined by phenotype microarray. Relative metabolic activity is expressed as percent of the mean metabolic activity of the mutants with respect to the wild type using triplicate experiments (Biolog Omnilog-PM software parameter average height). Shading refers to carbon sources whose uptake is not influenced (white bars), influenced in part (grey bars) or abolished (black bars) by mutation of ptsI .
Article Snippet: Metabolism of S. pneumoniae strains DP1004 and rough derivatives of G54 and TIGR4 were assayed by phenotype microarray (PM) microplates PM1 and PM2A (Biolog Inc, Hayward CA, USA) containing a total of 190 different carbon sources .
Techniques: Activity Assay, Microarray, Software, Mutagenesis